4HH4
Structure of the CcbJ Methyltransferase from Streptomyces caelestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97549 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 169.950, 244.000, 118.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.730 - 2.900 |
| R-factor | 0.18466 |
| Rwork | 0.183 |
| R-free | 0.22138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.730 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.079 | 0.472 |
| Number of reflections | 52391 | |
| <I/σ(I)> | 15.8 | 3 |
| Completeness [%] | 96.0 | 81.6 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Na HEPES pH 7.5, 1.3 M Li2SO4.H2O, 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
