4HH1
Dark-state structure of AppA wild-type without the Cys-rich region from Rb. sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 70.530, 70.530, 383.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.000 - 3.501 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22570 |
| Structure solution method | rigid body |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.036 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.154 | 0.836 |
| Number of reflections | 14223 | |
| <I/σ(I)> | 9.9 | 2.45 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.3M tris chloride buffer, 1.3M sodium chloride, 0.3M magnesium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
