4HGZ
Structure of the CcbJ Methyltransferase from Streptomyces caelestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97549 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.020, 244.550, 117.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.270 - 2.700 |
| R-factor | 0.18279 |
| Rwork | 0.181 |
| R-free | 0.22423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.270 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.071 | 0.472 |
| Number of reflections | 66712 | |
| <I/σ(I)> | 17.6 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Na HEPES pH 7.5, 1.3 M Li2SO4.H2O, 10% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
