4HGY
Structure of the CcbJ Methyltransferase from Streptomyces caelestis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97936, 0.97944, 0.97549 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.180, 245.140, 117.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.360 - 3.000 |
| R-factor | 0.22633 |
| Rwork | 0.225 |
| R-free | 0.24791 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.747 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BP3 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.400 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.378 |
| Number of reflections | 49290 | |
| <I/σ(I)> | 25.9 | 7.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.6 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Na HEPES pH 7.5, 1.3 M Li2SO4.H2O, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
