4HG4
Crystal structure of Fab 2G1 in complex with a H2N2 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.842, 133.143, 812.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.130 - 3.200 |
| R-factor | 0.2517 |
| Rwork | 0.249 |
| R-free | 0.30190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.366 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 6.780 | 3.150 |
| Rmerge | 0.133 | 0.070 | 0.819 |
| Number of reflections | 234983 | ||
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 98.7 | 98.5 | 95.6 |
| Redundancy | 6.6 | 6.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.7 | 10% PEG6000, 0.1 M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 295.7K |
