4HG2
The Structure of a Putative Type II Methyltransferase from Anaeromyxobacter dehalogenans.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.067, 88.447, 64.664 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 30.350 - 1.600 |
| R-factor | 0.1535 |
| Rwork | 0.152 |
| R-free | 0.17860 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.791 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.086 | 0.055 | 0.635 |
| Number of reflections | 71301 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.9 | 97.7 | 100 |
| Redundancy | 4.1 | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M MES pH 6.5, 12% PEG 20K, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
