4HFX
Crystal structure of a transcription elongation factor B polypeptide 3 from Homo sapiens, Northeast Structural Genomics consortium target id HR4748B.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 |
| Unit cell lengths | 80.643, 80.643, 63.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.800 - 2.540 |
| R-factor | 0.23634 |
| Rwork | 0.235 |
| R-free | 0.26283 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.060 | 0.377 |
| Number of reflections | 15239 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 97.6 | 81 |
| Redundancy | 7.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil method | 9 | 277 | 1.88M Na2s2o3, 0.1M TAPS PH9, Microbatch under oil method, temperature 277K |
