4HFV
Crystal structure of lpg1851 protein from Legionella pneumophila (putative T4SS effector)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794534 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 43.339, 43.339, 205.621 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.723 - 1.901 |
| R-factor | 0.1854 |
| Rwork | 0.183 |
| R-free | 0.23360 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.104 | 0.542 |
| Number of reflections | 18684 | |
| <I/σ(I)> | 13.4 | 2.06 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.2 M diammonium citrate, 20% PEG3350, 1/70 thermolysin, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
