4HFK

Crystal structure of the type VI effector-immunity complex Tae4-Tai4 from Enterobacter cloacae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	91.342, 138.144, 64.462
Unit cell angles	90.00, 127.89, 90.00

Refinement procedure

Resolution	23.402 - 2.100
R-factor	0.1568
Rwork	0.157
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hff
RMSD bond length	0.007
RMSD bond angle	1.057
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.232
Number of reflections	36726
<I/σ(I)>	34.5	10.5
Completeness [%]	100.0	100
Redundancy	7.7	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Sodium chloride, 0.1M Na/K phosphate pH 6.5, 25% (w/v) PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K