4HFD
The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to bromoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.218, 132.869, 159.376 |
| Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
| Resolution | 27.730 - 3.100 |
| R-factor | 0.1954 |
| Rwork | 0.195 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.105 | 0.653 |
| Number of reflections | 66928 | |
| <I/σ(I)> | 13.6 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 2% bromoform, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
