4HF8
Crystal structure of L-methionine gamma-lyase from Citrobacter freundii with glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-02 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.288, 123.052, 126.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.450 |
R-factor | 0.23 |
Rwork | 0.224 |
R-free | 0.33700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.730 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.450 | |
Number of reflections | 16559 | |
<I/σ(I)> | 7.79 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 5.04 | 3.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 5% MMEPEG 2000, 50MM TRIS-HCl, 0.2MM PLP, 0.25% DTT, 20mM glycine solution, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |