4HF1

Crystal Structure of IscR bound to its promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	49.124, 75.320, 187.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.081 - 2.222
R-factor	0.2047
Rwork	0.203
R-free	0.22770
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1161))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.220
Number of reflections	33154
<I/σ(I)>	10.6
Completeness [%]	94.0
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2-0.3M Ammonium Phosphate, 20% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K