4HEP
Complex of lactococcal phage TP901-1 with a llama vHH (vHH17) binder (nanobody)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.931 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 74.380, 74.380, 375.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.620 - 1.750 |
| R-factor | 0.1977 |
| Rwork | 0.198 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f0c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.029 | 0.460 |
| Number of reflections | 41184 | |
| <I/σ(I)> | 33 | 4.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1.6M ammonium sulfate, 0.1M MES, 6mg/ml protein complex, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
