4HEO
Hendra virus Phosphoprotein C terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 21.490, 43.280, 51.260 |
| Unit cell angles | 90.00, 79.78, 90.00 |
Refinement procedure
| Resolution | 32.848 - 1.650 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.22350 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.850 | 1.800 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.037 | 0.546 |
| Number of reflections | 21541 | |
| <I/σ(I)> | 14.24 | 1.89 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 2.7 | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Bis-Tris 100mM, PEG 3350 28%, MgCl2 50mM, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
