4HE6

Crystal structure of the C-terminal domain of Geobacillus thermoleovorans putative U32 peptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	28.400, 36.300, 65.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 1.100
R-factor	0.123
Rwork	0.123
R-free	0.16300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4he5
RMSD bond length	0.015
RMSD bond angle	2.330
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.800	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.116
Number of reflections	27941
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M sodium acetate, 0.2 M ammonium acetate, 30% (w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K