4HE0

Crystal structure of human muscle fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-15
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 42 21 2
Unit cell lengths	73.853, 73.853, 146.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.690
R-factor	0.202
Rwork	0.200
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyi
RMSD bond length	0.012
RMSD bond angle	1.393
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.790
High resolution limit [Å]	2.690	5.790	2.690
Rmerge	0.184	0.083	0.509
Number of reflections	11946
<I/σ(I)>	6.6
Completeness [%]	99.9	98.9	100
Redundancy	8.8	8.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M MgCl2, 15% PEG 4000, 0.1M Hepes pH 7.5, vapor diffusion, hanging drop, temperature 295K