4HDI

Crystal Structure of 3E5 IgG3 FAB from mus musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	67.070, 98.289, 142.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.760 - 2.449
R-factor	0.21134
Rwork	0.208
R-free	0.26348
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h1p 1t66
RMSD bond length	0.010
RMSD bond angle	1.430
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.449	2.449
Rmerge	0.082	0.535
Number of reflections	35380
<I/σ(I)>	20.8	3
Completeness [%]	99.9	100
Redundancy	6.5	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1