4HDE
The crystal structure of a SCO1/SenC family lipoprotein from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.985, 48.468, 88.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.560 - 1.317 |
| R-factor | 0.1629 |
| Rwork | 0.162 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.340 |
| High resolution limit [Å] | 1.317 | 1.317 |
| Rmerge | 0.105 | 0.653 |
| Number of reflections | 35792 | |
| <I/σ(I)> | 33.9 | 2.5 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.05M Magnessium chloride, 0.1M HEPES:NaOH, 30%(w/v)PEG MME550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
