4HD7
Crystal Structure of Tyrosinase from Bacillus megaterium V218G mutant soaked in CuSO4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Collection date | 2011-02-17 |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.900, 78.670, 85.770 |
| Unit cell angles | 90.00, 105.91, 90.00 |
Refinement procedure
| Resolution | 36.214 - 2.100 |
| R-factor | 0.2566 |
| Rwork | 0.254 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.214 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 82.484 | 41.242 | 2.210 |
| High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
| Rmerge | 0.036 | 0.181 | |
| Total number of observations | 2503 | 12298 | |
| Number of reflections | 34263 | ||
| <I/σ(I)> | 6.6 | 16.8 | 3.5 |
| Completeness [%] | 95.9 | 85.1 | 98.3 |
| Redundancy | 2.4 | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
