4HD4

Crystal Structure of Tyrosinase from Bacillus megaterium V218F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Collection date	2011-02-17
Wavelength(s)	0.976300
Spacegroup name	P 1 21 1
Unit cell lengths	48.000, 78.580, 85.890
Unit cell angles	90.00, 106.13, 90.00

Refinement procedure

Resolution	28.451 - 1.800
R-factor	0.2088
Rwork	0.207
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.222
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.530	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.099
Number of reflections	54388
Completeness [%]	96.3	96.3
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 8000, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 293K