4HCQ
Crystal structure of GLMU from mycobacterium tuberculosis in complex with glucosamine-1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-09 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.270, 79.270, 276.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.930 - 2.600 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dj4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.979 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.932 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.602 |
| Number of reflections | 19893 | |
| <I/σ(I)> | 20.1 | 2.86 |
| Completeness [%] | 99.4 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 8% PEG 8000, 150MM NACL, 5% GLYCEROL, 1,3-BUTANEDIOL, AMPPNP, MGCL2, 0.1M HEPES, COCL2, DTT, pH 7.5, VAPOR DIFFUSION, temperature 277K |
