4HCJ
Crystal Structure of ThiJ/PfpI Domain Protein from Brachyspira murdochii
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.799, 42.197, 66.366 |
| Unit cell angles | 90.00, 116.95, 90.00 |
Refinement procedure
| Resolution | 22.277 - 1.120 |
| R-factor | 0.116 |
| Rwork | 0.116 |
| R-free | 0.12700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | ahelx-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Number of reflections | 56517 | |
| <I/σ(I)> | 15 | 5.8 |
| Completeness [%] | 92.2 | 51.5 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Magnesium Chloride, 0.1 M HEPES:NaOH pH 7.5, 25% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
