4HCE

Crystal structure of the telomeric Saccharomyces cerevisiae Cdc13 OB2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1, 1.0005
Spacegroup name	P 21 21 21
Unit cell lengths	40.884, 78.770, 100.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.20438
Rwork	0.202
R-free	0.24671
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.553
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	14100
Completeness [%]	99.3	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M NaCl, 0.1 M BIS-Tris pH 6.5, and 1.5 M AmSO4, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M NaCl, 0.1 M BIS-Tris pH 6.5, and 1.5 M AmSO4, VAPOR DIFFUSION, SITTING DROP, temperature 291K