4HBR

Crystal structure of a putative periplasmic proteins (BACEGG_01429) from Bacteroides eggerthii DSM 20697 at 2.40 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-09
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	117.638, 117.638, 150.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.410 - 2.400
R-factor	0.1972
Rwork	0.196
R-free	0.21350
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.410	29.410	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge		0.063	0.722
Total number of observations		3576	25878
Number of reflections	47503
<I/σ(I)>	11.6	27.7	2.6
Completeness [%]	99.9	95	100
Redundancy	7.4	6.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.20M magnesium chloride, 10.00% polyethylene glycol 3000, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K