4HBL
Crystal structure of AbfR of Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.780, 50.616, 107.450 |
| Unit cell angles | 90.00, 110.39, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.21475 |
| Rwork | 0.210 |
| R-free | 0.25883 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 20535 | |
| Completeness [%] | 95.4 | 79.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 0.1M sodium acetate, 2.0M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
