4HBH
Bacterial Photosynthetic Reaction Center from Rhodobacter sphaeroides with ILE M265 replaced with ASN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-23 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.720, 139.720, 183.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 121.000 - 2.930 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.021 |
RMSD bond angle | 2.364 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 121.001 | 3.010 | |
High resolution limit [Å] | 2.930 | 13.100 | 2.930 |
Rmerge | 0.125 | 0.057 | 0.793 |
Number of reflections | 45001 | ||
<I/σ(I)> | 13.37 | 28.71 | 2.89 |
Completeness [%] | 99.6 | 69.1 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | drop: 13 mg/mL protein, 0.8 M potassium phosphate, 5 mM Tris, 5 uM EDTA, 4.0% 1,2,3-heptanetriol, 2.0% dioxane, 140 mM sodium chloride, 0.045% LDAO, reservoir: 1.6 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |