4HB9

Crystal structure of a putative FAD containing monooxygenase from Photorhabdus luminescens subsp. laumondii TTO1 (Target PSI-012791)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9790
Spacegroup name	P 32 2 1
Unit cell lengths	71.008, 71.008, 157.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.560 - 1.930
R-factor	0.1773
Rwork	0.175
R-free	0.21790
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.444
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHELX (C)
Refinement software	REFMAC (5.7.0025)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.930	1.930
Number of reflections	35439
<I/σ(I)>	30.5	5.38
Completeness [%]	100.0	99.3
Redundancy	19.2	18.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol, Reservoir (MCSG3 #31; C7: 0.1 M Bis-Tris Propane:HCl pH 7.0, 1.0 M Ammonium Citrate Tribasic pH 7.0 ); Cryoprotection (30% Ethylene glycol), VAPOR DIFFUSION, SITTING DROP, temperature 298K