4HB1

A DESIGNED FOUR HELIX BUNDLE PROTEIN.

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL
Synchrotron site	SSRL
Temperature [K]	100
Collection date	1995-05
Spacegroup name	P 64 2 2
Unit cell lengths	51.900, 51.900, 61.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.500 - 2.900
R-factor	0.234
Rwork	0.234
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODEL HELIX
RMSD bond length	0.020
RMSD bond angle	3.500
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.900	2.900
Rmerge	0.055 *
Total number of observations	19263 *
Number of reflections	1212 *
Completeness [%]	96.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.6		65% AMMONIUM SULPHATE, 3% ISOPROPANOL, 100MM TRIS, PH 8.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	65 (%sat)
2	1	reservoir	isopropanol	3 (%)
3	1	reservoir	Tris	100 (mM)
4	1	drop	ammonium sulfate	32.5 (%sat)
5	1	drop	Tris	50 (mM)
6	1	drop	DHP1	4 (mg/ml)