4HAS
Crystal structure of PX domain of human sorting nexin SNX27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.580, 47.820, 96.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.974 - 1.740 |
| R-factor | 0.1653 |
| Rwork | 0.163 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1065)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.970 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.098 | 0.816 |
| Number of reflections | 23145 | |
| <I/σ(I)> | 15.1 | 2.9 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 13.6 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 18% PEG 3.3k, 0.1M citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
