4HAG
Crystal structure of fc-fragment of human IgG2 antibody (centered crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-06 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.240, 144.400, 75.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.400 |
| R-factor | 0.30108 |
| Rwork | 0.299 |
| R-free | 0.34930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ave |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.084 | 0.341 |
| Number of reflections | 3896 | |
| <I/σ(I)> | 16.3 | 3.1 |
| Completeness [%] | 94.0 | 60.5 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 16% PEG 3350, 0.1 M HEPES. CRYO CONDITIONS: 23% PEG 3350, 0.1 M HEPES, 22% GLYCEROL, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
