4HAE
Crystal structure of the CDYL2-chromodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-29 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.358, 60.358, 89.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.100 - 2.000 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model of protein in slightly different crystal form itself based on molecular replacement with PDB entry 3LWE. |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.306 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.090 | 0.060 | 0.958 |
| Number of reflections | 7008 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.8 | 99.1 | 100 |
| Redundancy | 19.4 | 15.2 | 19.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 25% PEG3350, 0.2M magnesium chloride, 0.1M TRIS, pH 8.5, vapor diffusion, sitting drop, temperature 291K |
