4HAC
Crystal Structure of the Mevalonate Kinase from an Archaeon Methanosarcina mazei
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97947,0.97954,0.97179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.295, 135.359, 45.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.930 - 1.920 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.26280 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 5.180 | 1.910 |
| Rmerge | 0.093 | 0.074 | 0.709 |
| Number of reflections | 47072 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 97.8 | 94.1 | 72.2 |
| Redundancy | 6.9 | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | PEG 3350, MgCl2, pH 5.5, vapor diffusion, hanging drop, temperature 291K |
