4H9W
crystal structure of a METHIONINE mutant of WCI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 61.632, 61.632, 211.791 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
Rwork | 0.221 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eyl |
Data reduction software | AUTOMAR |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 | 0.055 |
Number of reflections | 8893 | |
<I/σ(I)> | 7.1 | 7.1 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 7.05 | 7.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.3M AMS, 5% GLYCEROL, 25% PEG 6K, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |