4H9R

Complex structure 5 of DAXX(E225A)/H3.3(sub5,G90A)/H4

Experimental procedure

Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	107.701, 107.701, 90.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.968 - 2.197
R-factor	0.1863
Rwork	0.184
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.197
Rmerge	0.071
Number of reflections	27814
<I/σ(I)>	33.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	1.6 M Na/K-phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K