4H9D

Crystal Structure of Mn-dependent Gme HNH nicking endonuclease from Geobacter metallireducens GS-15, Northeast Structural Genomics Consortium (NESG) Target GmR87

Replaces:  2QGP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	40.674, 83.385, 225.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.097 - 2.599
R-factor	0.2342
Rwork	0.233
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.297
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_988)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.600
Rmerge	0.082
Number of reflections	22466
<I/σ(I)>	29
Completeness [%]	98.7
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: MG-sulfate 0.2M, PEG 8000 20% (v/v), Tris-HCl 0.1M, VAPOR DIFFUSION, HANGING DROP, temperature 277K