4H8V
Crystal structure of the trehalulose synthase MUTB in complex with trehalulose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93400 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.270, 73.920, 82.960 |
| Unit cell angles | 66.07, 74.56, 72.55 |
Refinement procedure
| Resolution | 35.500 - 1.950 |
| R-factor | 0.1998 |
| Rwork | 0.197 |
| R-free | 0.25420 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zja |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 2.200 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.402 | |
| Number of reflections | 90958 | |
| <I/σ(I)> | 8.3 | 2.16 |
| Completeness [%] | 94.5 | 91.9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 12% PEG20000, 0.1M Na cacodylate, 0.01M L-cysteine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
