4H8U
MUTB inactive double mutant D200A-D415N soaked with sucrose and having as bound ligands sucrose in molecule A and the reaction product trehalulose in molecule B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.370, 73.680, 82.670 |
| Unit cell angles | 66.15, 74.29, 72.42 |
Refinement procedure
| Resolution | 38.400 - 2.000 |
| R-factor | 0.1922 |
| Rwork | 0.189 |
| R-free | 0.25670 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pwh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.313 | |
| Number of reflections | 83253 | |
| <I/σ(I)> | 9 | 2.69 |
| Completeness [%] | 94.2 | 94.2 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 20% PEG10000, 0.1M TRIS-HCL , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
