4H8Q
Structure of the Q29T IsdX2-NEAT5 mutant in complex with heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-06-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 109.090, 109.090, 109.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.569 - 1.700 |
R-factor | 0.1909 |
Rwork | 0.190 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h8p |
RMSD bond length | 0.006 |
RMSD bond angle | 1.056 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.570 |
High resolution limit [Å] | 1.490 |
Rmerge | 0.073 |
Number of reflections | 35031 |
Completeness [%] | 100.0 |
Redundancy | 44.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 293 | 5% PEG 3000, 0.2 M Zn acetate and 0.1 M sodium acetate pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |