4H8L

Crystal structure of a parallel 6-helix coiled coil CC-Hex-D24-A5/7C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 2 21
Unit cell lengths	31.540, 54.330, 98.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.031 - 1.750
R-factor	0.203
Rwork	0.200
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r46
RMSD bond length	0.015
RMSD bond angle	1.385
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.400
High resolution limit [Å]	1.750
Number of reflections	16619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	30 mM sodium nitrate, 30 mM disodium hydrogen phosphate, 30 mM ammonium sulfate, 100 mM bicine/Trizma base pH 8.5, 12.5 % PEG 1K, 12.5 % PEG 3350, 12.5 % MPD, VAPOR DIFFUSION, SITTING DROP, temperature 291K