4H8K
Crystal structure of LC11-RNase H1 in complex with RNA/DNA hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-13 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.613, 90.758, 55.605 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 38.280 - 2.300 |
| R-factor | 0.21025 |
| Rwork | 0.207 |
| R-free | 0.27785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u3g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.530 | 2.300 |
| Rmerge | 0.049 | 0.140 | 0.220 |
| Number of reflections | 17868 | ||
| <I/σ(I)> | 42.556 | 6.42 | 5.72 |
| Completeness [%] | 95.4 | 98 | 82.1 |
| Redundancy | 7.4 | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M ammonium acetate, 0.1M Tris, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
