4H7Z
tRNA-guanine transglycosylase Y106F, C158V mutant in complex with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2012-09-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.390, 64.750, 70.760 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 29.415 - 1.680 |
| R-factor | 0.1587 |
| Rwork | 0.157 |
| R-free | 0.19110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GEV |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.070 | 0.048 |
| Number of reflections | 45941 | |
| <I/σ(I)> | 10.2 | 2.1 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Tris, 1 mM DTT, 10% DMSO, 12% PEG 8000 , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
