4H7W
Crystal Structure of Human C16orf57
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-13 |
| Detector | ADSC |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.247, 51.070, 48.013 |
| Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
| Resolution | 45.810 - 1.100 |
| R-factor | 0.13276 |
| Rwork | 0.132 |
| R-free | 0.15434 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.225 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.810 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.076 | 0.324 |
| Number of reflections | 79339 | |
| <I/σ(I)> | 12 | 2.3 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 25% PEG 4000, 0.2 M ammonium sulphate, 0.1 M Na citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
