4H7V
MUTB inactive double mutant D200A-D415N in complex with GLUCOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-15 |
| Detector | PILATUS 6M |
| Wavelength(s) | 0.98081 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.700, 71.200, 85.400 |
| Unit cell angles | 118.10, 90.20, 109.70 |
Refinement procedure
| Resolution | 42.200 - 1.800 |
| R-factor | 0.1737 |
| Rwork | 0.171 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zjb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.308 | |
| Number of reflections | 105583 | |
| <I/σ(I)> | 8.4 | 3.4 |
| Completeness [%] | 92.9 | 92.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 20% PEG10000, 0.1M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
