4H7P
Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.580, 65.660, 77.650 |
| Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
| Resolution | 28.920 - 1.300 |
| R-factor | 0.1534 |
| Rwork | 0.152 |
| R-free | 0.17850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iz9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
| Rmerge | 0.047 | 0.029 | 0.336 |
| Number of reflections | 146529 | 1567 | 10351 |
| <I/σ(I)> | 24.71 | 59.37 | 4.11 |
| Completeness [%] | 97.9 | 88.6 | 94 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | EmeraldBio JCSG+ screen h7: 25% PEG 3350, 200mM ammonium sulphate, 100mM BisTris pH 5.5; LemaA.01212.a.A1.P001468 at 20mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
