4H7O
Crystal structure of Serine acetyltransferase from Vibrio cholerae O1 biovar El Tor N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.953 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.040, 75.040, 73.430 |
| Unit cell angles | 90.00, 89.04, 90.00 |
Refinement procedure
| Resolution | 37.520 - 2.170 |
| R-factor | 0.17957 |
| Rwork | 0.177 |
| R-free | 0.21998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.104 | 0.356 |
| Number of reflections | 38412 | |
| <I/σ(I)> | 5.06 | |
| Completeness [%] | 97.4 | 87.4 |
| Redundancy | 7.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 10% Peg4000, 20% Glycerol, 0.03M MgCl2,CaCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
