4H6Z
Tubulin acetyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9806, 0.9637 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.488, 81.488, 117.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.710 - 2.701 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.23820 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11419 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH 6.0, 0.6 M NaH2PO4 and KH2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
