4H6V
Structure of Patellamide maturation protease PatA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2009-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 83.717, 83.717, 42.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.2116 |
| Rwork | 0.210 |
| R-free | 0.24350 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.083 | 0.048 | 0.661 |
| Number of reflections | 31792 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 97.4 | 99.8 | 75.6 |
| Redundancy | 12.2 | 14.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281 | PEG 20000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
