4H6U

Tubulin acetyltransferase mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	58.128, 58.128, 225.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.064 - 2.448
R-factor	0.199
Rwork	0.196
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h6z
RMSD bond length	0.015
RMSD bond angle	1.524
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	15066
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.65M NaH2PO4 and KH2PO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K