4H63
Structure of the Schizosaccharomyces pombe Mediator head module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99977 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 145.600, 145.600, 241.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 126.093 - 3.400 |
| R-factor | 0.2332 |
| Rwork | 0.232 |
| R-free | 0.25790 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.093 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.125 | 0.041 |
| Number of reflections | 41454 | |
| <I/σ(I)> | 15.16 | 0.64 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50 mM MES, 1 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.0 |
