4H5Q

Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Hexamer Bound to Single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 64 2 2
Unit cell lengths	108.640, 108.640, 261.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.660 - 2.700
R-factor	0.1911
Rwork	0.189
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lyf
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.085	53.658	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.031	0.779
Total number of observations		3587	15490
Number of reflections	25771
<I/σ(I)>	10	16.8	1
Completeness [%]	99.6	95.5	98.5
Redundancy	4.4	4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	15% PEG3350, 350 mM sodium chloride, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K